Full LDH was made by incorporating lysis buffer to liberate all cytoplasmic LDH. Cell viability was calculated with percentage of whole LDH. With the PI staining, Cells had been handled with indicated stimulus and then PI (5 μg/mL) was instantly added to medium and incubated for 20 min. The pictures https://lorenzopizri.buyoutblog.com/18613337/details-fiction-and-hg-9-91-01